Detail

Localization	Tissue	Validated Method	PMID
Axon	Dorsal root ganglia	RT-PCR	15673657
Description: By RT-PCR, we were able to detect peripherin, vimentin, and cofilin mRNAs in DRG axonal preparations (Fig. 4A). By RT-PCR, rTpm3 mRNA was detected in axonal preparations from the cultured DRGs (Fig. 4A). alpha-B crystallin and HSP70 mRNAs appeared more concentrated in the axonal than in the cellbody RNA preparations. HSP60 and HSP90 mRNAs show relatively equal levels in the axonal and cell-body RNAs, whereas HSP27 and grp75 appear more concentrated in the cell-body than in the axonal RNAs. Resident-ER proteins and encoding mRNAs in DRG axons. A, RT-PCR for rat grp78/BiP, calreticulin, and ERp29 mRNAs shows that these transcripts extend into the DRG axons. Ubiquitin C-terminal hydrolase L1 (Uch-L1), SP22, r-synuclein, and dismutase (SOD1) were detected by the MS analyses, and RT-PCR confirmed that the mRNAs encoding each of these extends into the DRG axons (Fig. 7A).

Other Subcellular Localization:

RLID	Localization	Tissue	Validated Method	PMID
RLID00002513	Axon	Dorsal root ganglia	qRT-PCR	17785519