Detail
| RLID: |
RLID00002913 |
| RNA Symbol: |
Cebpb |
| RNA Category: |
mRNA |
| Organism: |
Mus musculus |
| Aliase: |
C/EBPbeta, CRP2, IL-6DBP, LAP, LIP, NF-IL6, NF-M, Nfil6 |
| Homology: |
CEBPB (Homo sapiens
) |
| Subcellular Localization: |
| Localization |
Tissue |
Validated Method |
PMID |
| Nucleus |
betawt|betad/s cell |
RT-PCR |
18678862 |
|
Description: To investigate the influence of HuR on nuclear processing of the C/EBPb mRNA, we examined nuclear polyadenylation of the C/EBPb transcripts from the betawt and betad/s cell lines. To accumulate the data presented in Fig. 7, RNA was isolated from the nuclear fraction and subjected to reverse transcription using an oligo(dT) primer/adapter followed by PCR using a forward primer located 298 nucleotides upstream of the site of poly(A) addition of the C/EBPb mRNA in conjunction with the n32P-labeled oligo(dT) primer/adapter. The data suggest that in the absence of HuR binding, the C/EBPb mRNA is more extensively polyadenylated, leading to translocation to the cytosol. However, in betawt, which binds HuR, polyadenylation appears to occur to a lesser extent, with approximately one-third of the RNA (relative to betad/s) reaching the cytosol.
|
Other Subcellular Localization: