Detail

RLID: RLID00003617
RNA Symbol: Gapdh
RNA Category: mRNA
Organism: Mus musculus
Aliase: Gapd
Sequence:
Homology: GAPDH (Homo sapiens), Gapdh (Rattus norvegicus )
Subcellular Localization:
Localization Tissue Validated Method PMID
Ribosome B cell Northern blot 12923260
Description: In this report, we use multiple cell fractionation protocols, in combination with cDNA microarray, nuclease protection, and Northern blot analyses, to assess the distribution of mRNAs between free and ER-bound ribosomes. We find a broad representation of mRNAs encoding soluble proteins in the ER fraction, with a subset of such mRNAs displaying substantial ER partitioning. In addition, we present evidence that membrane-bound ribosomes engage in the translation of mRNAs encoding soluble proteins. Single-cell in situ hybridization analysis of the subcellular distribution of mRNAs encoding ER-localized and soluble proteins identify two overall patterns of mRNA distribution in the cell-endoplasmic reticular and cytosolic. FIGURE 4. Steady-state mRNA partitioning between cytosolic and ER-bound polysomes. Total RNA was isolated from cytosol and ER membrane fractions of Jurkat and J558 cells.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00003615 Cytoplasm Fibroblast qRT-PCR|Northern blot 16874305
RLID00003616 Exosome Mast cell Microarray 17486113
RLID00003618 Ribosome B cell S1 nuclease protection assays 12923260
RLID00003619 Cytosol Macrophage qRT-PCR 26179904
RLID00003620 Axon Motoneuron In situ hybridization|qRT-PCR 26464439
RLID00003621 Somatodendritic compartment Motoneuron In situ hybridization|qRT-PCR 26464439
RLID00003622 Nucleus 293T cell qRT-PCR 23898399
RLID00003623 Cytoplasm 293T cell qRT-PCR 23898399
RLID00003624 Ribosome J558 cell Northern blot 12923260