Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Ribosome |
J558 cell |
Northern blot |
12923260 |
|
Description: FIGURE 4. Steady-state mRNA partitioning between cytosolic and ER-bound polysomes. Total RNA was isolated from cytosol and ER membrane fractions of Jurkat and J558 cells, as indicated in Figure 1A. For each reaction, 10 ug of RNA was incubated with one of eight [32P]-labeled oligonucleotide probes representing three different classes of genes, based on the type of protein encoded: cytosolic, ER membrane, and ER lumenal. The nuclease-protected RNA fragments from parallel reactions with cytosol and ER-derived RNA were purified and separated by denaturing acrylamide gel electrophoresis. Quantification was performed by phosphorimager analysis; photostimulated luminescence output (PSL) values are indicated. Digital images of the phosphorimager output are depicted. The data are collected from Figure 4.
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Other Subcellular Localization: