Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Endoplasmic reticulum |
NIH-3T3 Fibroblast |
In situ hybridization |
12923260 |
|
Description: For these experiments, three mRNAs were examined: that encoding GRP94, which is highly enriched in the ER membrane fraction of fractionated cells; GAPDH, which displays a predominately (but not uniquely) soluble enrichment; and Hsp90, which displays noncanonical partitioning on the ER. Experiments were performed using NIH-3T3 fibroblasts and digoxygenin-labeled antisense RNA probes, with optical sections obtained by widefield microscopy.As expected, mRNAs encoding GRP94 display a typical, perinuclear and reticular ER pattern (Fig. 6A), with the ImageJ rendering providing a graphical display of the perinuclear, ER partitioning of this mRNA species (Fig. 6A, far right panel).
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Other Subcellular Localization: