Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Axon |
Neuron |
Fluorescence in situ hybridization |
20308067 |
|
Description: MRNAs encoding the endoplasmic reticulum chaperone proteins calreticulin and Grp78/BiP were previously detected in sensory axons by RT-PCR and array hybridization (7, 30). Because these analyses used lysed axons that could contain proximal axonal segments, we used FISH to directly visualize the mRNAs in dissociated cultures of DRG neurons. DRG neurons showed punctate FISH signals for beta-actin, calreticulin, and Grp78/BiP mRNAs that extended into growth cones (Fig. 1A). This granular distribution is similar to other mRNAs localizing to dendrites and axons (21).mRNAs encoding the endoplasmic reticulum chaperone proteins calreticulin and Grp78/BiP were previously detected in sensory axons by RT-PCR and array hybridization (7, 30). Because these analyses used lysed axons that could contain proximal axonal segments, we used FISH to directly visualize the mRNAs in dissociated cultures of DRG neurons. DRG neurons showed punctate FISH signals for beta-actin, calreticulin, and Grp78/BiP mRNAs that extended into growth cones (Fig. 1A). This granular distribution is similar to other mRNAs localizing to dendrites and axons (21).
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Other Subcellular Localization: