Detail
| RLID: | RLID00008374 |
| RNA Symbol: | GAPDH |
| RNA Category: | mRNA |
| Organism: | Homo sapiens |
| Aliase: | G3PD, GAPD, HEL-S-162eP |
| Homology: | Gapdh (Mus musculus), Gapdh (Rattus norvegicus ) |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Endoplasmic reticulum | U2OS cell | Fluorescence in situ hybridization | 26272916 |
| Description: To determine whether partial ER association was a general phenomenon for all mRNAs, we next investigated the localization of an mRNA encoding a cytosolic protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH). We could reproducibly find 15% of the GAPDH puncta in digitonin-extracted cells (Fig. 1A,B). However, in contrast to what we had seen for Sec61b and nesprin-2, most of the GAPDH mRNAs were extracted in cells treated with either HHT, or puromycin+EDTA (Fig. 1), suggesting that the small amount of ER association was mediated by translating ribosomes. Data are collected from Figure 1. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00008363 | Ribosome | Jurkat cell | Northern blot | 12923260 |
| RLID00008364 | Ribosome | T-cell|SeAx | qRT-PCR | 19175411 |
| RLID00008365 | Ribosome | Colon cancer cell | Next-generation sequencing | 24393600 |
| RLID00008366 | Exosome | Colon|Liver|Lung | qRT-PCR | 22895844 |
| RLID00008367 | Ribosome | T-cell line Jurkat | Northern blot | 12923260 |
| RLID00008368 | Ribosome | T-cell line Jurkat | S1 nuclease protection assays | 12923260 |
| RLID00008369 | Mitochondrion | HDF cell | Fluorescence in situ hybridization | 16178658 |
| RLID00008370 | Cytosol | HEK293 cell | Northern blot | 21431749 |
| RLID00008371 | Endoplasmic reticulum | HEK293|HeLa|HDF|J558 cell | qRT-PCR | 25142066 |
| RLID00008372 | Cytosol | HeLa cell|HEK293 cell | In situ hybridization | 25142066 |
| RLID00008373 | Ribosome | HEK293|HeLa|HDF|J558 cell | qRT-PCR | 25142066 |
| RLID00008375 | Ribosome | HEK293 cell | Next-generation sequencing | 22199352 |
| RLID00008376 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00008377 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00008378 | Cytoplasm | Embryonic stem cell | qRT-PCR|Microarray | 22193719 |
| RLID00008379 | Cytosol | HT1080 cell | Fluorescence in situ hybridization|qRT-PCR | 25753659 |