Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Endoplasmic reticulum |
COS-7 cell |
Fluorescence in situ hybridization |
24019514 |
|
Description: COS-7 cells were transfected with plasmid containing either full-length ALPP, t-ftz, AP1, AP2, AP3, AP4, or AP5 and allowed to express mRNA for 18-24 h. The cells were then treated with DMSO (C or Ctrl) or HHT (H) for 30 min and then extracted with digitonin. Cells were then fixed, stained for mRNAs using specific FISH probes (ftz probe was used to detect AP1-5), and imaged. Figure 3A, quantification of the fluorescence intensities of mRNA in the ER and nucleus. Data are collected from Figure 3.
|
Other Subcellular Localization: