Detail
| RLID: |
RLID00011469 |
| RNA Symbol: |
ITGB1 |
| RNA Category: |
mRNA |
| Organism: |
Homo sapiens |
| Aliase: |
CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB |
| Homology: |
Itgb1 (Mus musculus
) |
| Subcellular Localization: |
| Localization |
Tissue |
Validated Method |
PMID |
| Endoplasmic reticulum |
U2OS cell |
RT-PCR |
22679391 |
|
Description: Endogenous mRNAs remain associated with the ER independently of ribosomes and translation.(A) The levels of Sec61a and bactin transcripts isolated from unbound (i.e., non-ER) cytoplasmic and ER fractions from either cycloheximide treated (“Controlâ€) or puromycin treated EDTA extracted ( Puromycin+EDTA ) U2OS cells were assessed by quantitative RT-PCR analysis. Each bar represents the levels of the specified transcript normalized to 28S rRNA levels, standardized to the level of mRNA in the control sample, and averaged between three independent experiments. Error bars represent the standard error of the mean. Note that 28S rRNA was used as the large ribosomal subunit is known to associate to the ER even after puromycin treatment [35] and that ribosomes are equally distributed in cytoplasmic and ER (see Figure 1F). The level of Sec61a mRNA was normalized to the ER fraction from control cells, while the b-actin mRNA was normalized to the cytoplasmic fraction from control cells. (B) The levels of several transcripts in the ER fraction were analyzed as in (A). Measured transcripts include those encoding ER luminal proteins (BiP, Calreticulin), ER membrane proteins (Inositol-3-phosphate Receptor (IP3 Receptor), Sec61a, Trapa, and Fatty Acid Desaturase 3 (FADS3)), a Golgi protein (Mannosidase 2A (Man2A)), plasma membrane proteins (Integrin b1, and Transferrin Receptor (Tf Receptor)), and a secreted protein (Interleukin 7 (IL7)). All measurements were standardized to the level of mRNA in the ER fraction from control cells.
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Other Subcellular Localization: