Detail
| RLID: | RLID00018182 |
| RNA Symbol: | ACTB |
| RNA Category: | mRNA |
| Organism: | Homo sapiens |
| Aliase: | BRWS1, PS1TP5BP1 |
| Homology: | ACTB (Gallus gallus), Actb (Mus musculus) |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Ribosome | HT1080 cell | qRT-PCR | 25753659 |
| Description: Figure S1: Polysomal gradient analysis. HT1080 cells were incubated under control (21% oxygen) or hypoxic (1% oxygen) conditions for up to 36 h as described in Figure 1. Shown are representative original RT-PCR data (30 cycles for beta-Actin and the external standard [extSt]; 35 cycles for the other genes) from pooled samples to visualize mRNA distribution following fractionation of sucrose gradients at control conditions (C) and 36 h of hypoxia (Hy). The external standard (a synthetic in vitro transcript) was diluted to appropriate concentration for qPCR and added directly after gradient fractionation and prior to RNA isolation as a technical control. The external standard served for fraction dependent normalization. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00018166 | Ribosome | Colon cancer cell | Next-generation sequencing | 24393600 |
| RLID00018167 | Exosome | Colon|Liver|Lung | qRT-PCR | 22895844 |
| RLID00018168 | Cell leading edge | Jurkat cell | In situ hybridization | 12923260 |
| RLID00018169 | Nucleus | HeLa cell | In situ hybridization | 15081402 |
| RLID00018170 | Cell leading edge | SW480 CRC cell | Fluorescence in situ hybridization|qRT-PCR | 18535985 |
| RLID00018171 | Cytosol | NCI/ADR-RES cell | qRT-PCR | 20453113 |
| RLID00018172 | Lamellipodium | Lung fibroblast | Fluorescence in situ hybridization | 23452202 |
| RLID00018173 | Cell leading edge | Lung fibroblast | qRT-PCR | 23452202 |
| RLID00018174 | Mitochondrion | HeLa cell | Sticky-Flare Synthesis | 26195734 |
| RLID00018175 | Ribosome | Kidney | RT-PCR | 26239904 |
| RLID00018176 | Ribosome | HEK293 cell | Next-generation sequencing | 22199352 |
| RLID00018177 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00018178 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00018179 | Cytoplasm | Embryonic stem cell | qRT-PCR|Microarray | 22193719 |
| RLID00018180 | Cytoplasm | U2OS cell | RT-PCR | 22679391 |
| RLID00018181 | Cytosol | HT1080 cell | Fluorescence in situ hybridization|qRT-PCR | 25753659 |
| RLID00018183 | Ribosome | Lung fibroblast | qRT-PCR | 23452202 |