Detail

Localization	Tissue	Validated Method	PMID
Nucleus	Brain	Microarray	22955988
Description: We examined the subcellular location of a number of well-known lncRNAs (Fig. 8D). Unsurprisingly, the X-chromosome inactivating transcript XIST was extremely highly enriched in the nucleus for all cells we examined (with a maximum enrichment of 273-fold in the nucleus of GM12878 cells) (Fig. 8D). Other regulatory lncRNAs such as GAS5, LINC00568 (also known as ncRNA-a1), CYP4A22-AS1 (also known as ncRNA-a3), MIAT, and MEG3 were nuclear enriched in at least two different cell types, consistent with their reported roles in gene regulation. Other transcripts, including the bifunctional transcript SRA1, which acts as both a regulatory RNA and a protein-coding sequence, have more variable subcellular location depending on cell type. As reported previously, the H19 transcript is consistently enriched in the cytoplasm, especially when comparing with the chromatin fraction (cytoplasmic/chromatin enrichment 167-fold).

Other Subcellular Localization:

RLID	Localization	Tissue	Validated Method	PMID
RLID00018108	Nucleus	HeLa cell	Fluorescence in situ hybridization	20124551
RLID00018109	Nucleus	Colon cancer cell	Next-generation sequencing	24393600
RLID00018110	Cytoplasm	HeLa cell	Fluorescence in situ hybridization	20124551
RLID00018111	Cytoplasm	Hepatic stellate cell	qRT-PCR	26446789
RLID00018113	Cytoplasm	Foreskin fibroblast	Florescence micrograph	25630241
RLID00018114	Nucleus	Foreskin fibroblast	Florescence micrograph	25630241
RLID00018115	Nucleus	Hela cell	qRT-PCR\|Fluorescence in situ hybridization	25630241
RLID00018116	Nucleus	Lung fibroblast	qRT-PCR\|Fluorescence in situ hybridization	25630241