Detail

Localization	Tissue	Validated Method	PMID
Vegetal	Oocyte	In situ hybridization	9148809
Description: Figure 3 Vg1 mRNA localization involves an interaction with the ER. (A) Vera cross-linking activity cosediments with ER membranes. Cytoplasmic extracts from pooled oocytes (stage IV through VI) were fractionated by sucrose density gradient sedimentation. (D) Endogenous Vg1 mRNA is associated with a subcompartment of the ER during localization. Endogenous Vg1 mRNA is distributed throughout the cytoplasm of early stage II oocytes (Fig. 3D), but at the transition between stages II and III (II/III) of oogenesis, Vg1 mRNA accumulates in a wedge-shaped region between the nucleus and vegetal cortex (Fig.3D) (5). As oogenesis proceeds to stage III, Vg1 mRNA labeling accumulates at the vegetal cortex (Fig. 3D) (5, 12,13). Before localization, Vg1 mRNA and the ER are probably not associated, because in stage II oocytes the Vg1 mRNA distribution is punctate and the ER is reticular (Fig. 3D).

Other Subcellular Localization: