Detail

RLID: RLID00019948
RNA Symbol: gdf1
RNA Category: mRNA
Organism: Xenopus laevis
Aliase: dvr-1, dvr1, gdf-1, vg1
Sequence:
Subcellular Localization:
Localization Tissue Validated Method PMID
Vegetal Oocyte In situ hybridization 9148809
Description: Figure 3 Vg1 mRNA localization involves an interaction with the ER. (A) Vera cross-linking activity cosediments with ER membranes. Cytoplasmic extracts from pooled oocytes (stage IV through VI) were fractionated by sucrose density gradient sedimentation. (D) Endogenous Vg1 mRNA is associated with a subcompartment of the ER during localization. Endogenous Vg1 mRNA is distributed throughout the cytoplasm of early stage II oocytes (Fig. 3D), but at the transition between stages II and III (II/III) of oogenesis, Vg1 mRNA accumulates in a wedge-shaped region between the nucleus and vegetal cortex (Fig.3D) (5). As oogenesis proceeds to stage III, Vg1 mRNA labeling accumulates at the vegetal cortex (Fig. 3D) (5, 12,13). Before localization, Vg1 mRNA and the ER are probably not associated, because in stage II oocytes the Vg1 mRNA distribution is punctate and the ER is reticular (Fig. 3D).

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00019946 Cytoskeleton Oocyte In situ hybridization 8223259
RLID00019947 Cytoplasm Oocyte In situ hybridization 9148809
RLID00019949 Mitochondrion Oocyte In situ hybridization 9545550
RLID00019950 Cytoplasm Oocyte In situ hybridization 9545550
RLID00019951 Vegetal Oocyte Immunofluorescence 15496522
RLID00019952 Vegetal Oocyte In situ hybridization 26337391
RLID00019953 Vegetal Oocyte In situ hybridization 11309203
RLID00019954 Endoplasmic reticulum Oocyte In situ hybridization 9545550
RLID00019955 Vegetal Oocyte In situ hybridization|RT-PCR 11784070
RLID00019956 Germ plasm Oocyte Microscopy 23626739