Detail
| RLID: |
RLID00024121 |
| RNA Symbol: |
SEC4 |
| RNA Category: |
mRNA |
| Organism: |
Saccharomyces cerevisiae |
| Aliase: |
YFL005W, SRO6 |
| Homology: |
RAB8A (Homo sapiens
) |
| Subcellular Localization: |
| Localization |
Tissue |
Validated Method |
PMID |
| Cellular bud |
Yeast |
Fluorescence in situ hybridization |
17339339 |
|
Description: As most POL proteins are enriched in the bud, we examined the localization of their mRNAs during cell division by FISH with specific digoxigenin-labeled antisense probes (Fig. 1). First, we examined the localization of endogenous mRNA encoding Sec4 (Fig. 1A), a bud-localized GTPase that mediates secretory vesicle fusion with the plasma membrane, in WT cells. FISH labeling of endogenous SEC4 mRNA yielded single punctate structures located near the cell surface prior to budding (G1?phase). These structures were distinct from the nucleus, which was revealed by propidium iodide staining (Fig. 1A). In cells undergoing division (early G2/M phase), nearly all SEC4 mRNA was found in the bud even though nuclear division and segregation had yet to occur. This pattern was observed in 85% of the cells examined. Cells in late G2/M also had SEC4 mRNA located in the nucleus of the mother cell. This may represent a maternal pool ofSEC4 mRNA yet to be exported. After mitosis, SEC4 mRNA was observed in both mother and daughter cells.
|
Other Subcellular Localization: