Detail

Localization	Tissue	Validated Method	PMID
Cellular bud	Yeast	Fluorescence in situ hybridization	17339339
Description: As SEC4 mRNA is asymmetrically distributed to the bud prior to nuclear division, similar to ASH1 mRNA, we examined the localization of other POL mRNAs by FISH (Fig. 1B). Endogenous mRNAs encoding the Cdc42 and Rho3 GTPases (31); the Sec1, Sro7, and Sro77 SNARE regulators (23,25); the Sec3 and Exo84 exocyst components (27); and Ypt1, a GTPase involved in ER-Golgi transport, localized at least in part to the buds of G2/M phase cells prior to nuclear division. This pattern was discerned in éˆ®?0% of cells for each mRNA examined (Fig. 1B), indicating that these mRNAs were also exported from mother cells. Asymmetric POL mRNA localization correlates with the bud-specific pattern of protein localization seen during cell division.

Other Subcellular Localization:

RLID	Localization	Tissue	Validated Method	PMID
RLID00024186	Mitochondrion	Yeast	Microarray	11818335
RLID00024188	Endoplasmic reticulum	Yeast	RT-PCR	17339339
RLID00024189	Cellular bud	Yeast	Fluorescence in situ hybridization	17339339
RLID00024190	Cytosol	Yeast	RT-PCR	23904265