Detail

Localization	Tissue	Validated Method	PMID
Endoplasmic reticulum	Yeast	RT-PCR	19903887
Description: To further analyze the specificity of mPP targeting, we examined the localization of the POX1 and GPD1 mRNAs in cells expressing Sec63-RFP (Fig. S1 B and C and Table S3). Interestingly, a high percentage of both RNAs colocalized with Sec63-RFP (59% and 71%, respectively; Table S3 and Fig. S1B and C). While mRNA localization to the ER could be due to the fact that ER fills much of the cell volume (see, e.g., Fig. S1B and C), we also demonstrated that peroxisomes decorate both nuclear and cortical ER (Fig. S1D). Thus, discerning whether mPP localization to the ER results from a direct association with ER membranes or is a consequence of binding to ER-associated peroxisomes is difficult. Alternatively, GPD1 mRNA, which encodes a glycerol-3-phosphate dehydrogenase found in the cytosol and peroxisomes, may preferentially localize to ER-like mRNAs encoding cytoplasmic proteins (13). This could allow for protein distribution to either compartment. Data are collected from Table S3: Localization of mPPs to ER.

Other Subcellular Localization: