Detail

Localization	Tissue	Validated Method	PMID
Endoplasmic reticulum	Yeast	Confocal microscopy	23904265
Description: We examined mRNA localization by confocal microscopy and found that 9 of 11 mSMPs tagged (ALG1, GOS1, ICE2, PEP12, PMT2, SUC2, SUR4, USE1, and YIP3) showed high levels of granule colocalization with ER (>70%; Figure 1 and Table 1), and 2 (NYV1 and SEC22) showed lower levels of ER localization (56%; Figure 1 and Table 1). A similar low level of ER localization was observed with two mRNAs that encode soluble SNAREs (SEC9 and SEC20) known to associate with the plasma and ER membranes (Figure 1 and Table 1). Of interest, some preference toward nER localization was observed with mSMPs such as PMT2, SUC2, SUR4, USE1, and YIP3, whereas in contrast, most of the mRNAs encoding SNAREs (e.g., GOS1, NYV1, PEP12, SEC9, and SEC22) demonstrated a preference for cER (Table 1). Data are collected from Table 1.

Other Subcellular Localization: