Detail

Localization	Tissue	Validated Method	PMID
Cellular bud	Yeast	Fluorescence in situ hybridization	17094940
Description: As described above, the long sequences of these mRNAs (3.4 kb ORF for CSR2 and 2.9 kb ORF for DAL81) might generate signals of the nucleus. To confirm that, 30 -tagged mRNAs, CSR2-U1A and DAL81-U1A, were constructed by insertion of the 4è·¯ U1A-tag between their ORF and 30-UTR. CSR2-U1A and DAL81-U1A were expressed from the GAL1 promoter and subjected to the U1A-tag-GFP system. They were detected at the bud-tip but not in the nucleus (Fig. 3A, c and g), whereas signals of both the 50-tagged and the 30-tagged mRNAs were detected in the nucleus and at the bud-tip by in situ hybridization (Fig. 3A, FISH column). These results suggested that CSR2 and DAL81 mRNAs were localized at the bud-tip after transcription.

Other Subcellular Localization:

RLID	Localization	Tissue	Validated Method	PMID
RLID00024827	Mitochondrion	Yeast	Microarray	11818335