Detail
| RLID: |
RLID00027689 |
| RNA Symbol: |
hsa-mir-425 |
| RNA Category: |
miRNA |
| Organism: |
Homo sapiens |
| Subcellular Localization: |
| Localization |
Tissue |
Validated Method |
PMID |
| Exosome |
Plasma |
qRT-PCR |
24937531 |
|
Description: For miRNome analysis, two homogeneous samples were created: one by pooling 2 æ¸L of RNA from exosomes of diabetic patients and the other by pooling RNA from exosomes of control subjects. After reverse transcription (mirCURY LNA universal cDNA synthesis kit; Exiqon), the reaction was performed on a serum/plasma focus miRNA PCR panel 384 well (V1.R) (Exiqon) using the ABI 7900HT (Applied Biosystems). The most stable miRNAs (miR-425 and miR-423-5p) were identified by NormFinder and GeNorm, validated on each sample, and used as normalizators for circulating miRNAs.
|
Other Subcellular Localization: