Detail
| RLID: | RLID00027929 |
| RNA Symbol: | hsa-let-7a-5p |
| RNA Category: | miRNA |
| Organism: | Homo sapiens |
| Aliase: | hsa-let-7a |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Exosome | Serum | RT-PCR | 22427800 |
| Description: The miRNAs tested in serum and saliva samples are shown in Table 1. These miRNAs were selected because they are either ubiquitously expressed or have been reported as biomarkers (Table 1). The relative concentration of these microRNAs was determined by calculating the difference of Ct values between the exosome samples and the exosome-depleted supernatant. A 1-unit difference in the Ct value between the miRNAs isolated from the exosomal pellet or supernatant represents a 2-fold difference in the amount of input miRNA. Data are collected from Table 1: List of miRNAs tested in serum and saliva samples. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00027918 | Circulating | Plasma | qRT-PCR | 22262318 |
| RLID00027919 | Circulating | Cerebrospinal fluid | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00027920 | Circulating | Serum | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00027921 | Circulating | Serum | Next-generation sequencing | 23641832 |
| RLID00027922 | Circulating | Plasma | qRT-PCR | 24937531 |
| RLID00027923 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00027924 | Cytoplasm | HeLa cell | Microarray | 21695135 |
| RLID00027925 | Exosome | Serum | Microarray | 18589210 |
| RLID00027926 | Exosome | Breast cancer cell | Microarray | 20976003 |
| RLID00027927 | Exosome | Dendritic cell | Microarray | 21505438 |
| RLID00027928 | Exosome | B cell | Microarray | 21505438 |
| RLID00027930 | Exosome | Brain tissue | Microarray | 23382797 |
| RLID00027931 | Exosome | Plasma | Next-generation sequencing | 23663360 |
| RLID00027932 | Exosome | Breast cancer cell | RT-PCR|Microarray | 24468161 |
| RLID00027933 | Exosome | Esophageal cancer cell | Next-generation sequencing | 25184951 |
| RLID00027934 | Microvesicle | Plasma | qRT-PCR | 19002258 |
| RLID00027935 | Microvesicle | Glioblastoma cell | qRT-PCR|Microarray | 19011622 |
| RLID00027936 | Microvesicle | Plasma | Next-generation sequencing | 23077538 |
| RLID00027937 | Microvesicle | Seminal Plasma | Microarray | 23539611 |
| RLID00027938 | Microvesicle | Hepatoma cell | qRT-PCR|Microarray | 23771658 |
| RLID00027939 | Mitochondrion | HeLa cell | Next-generation sequencing | 22984580 |
| RLID00027940 | Mitochondrion | Eembryonic kidney cell | Next-generation sequencing | 22984580 |
| RLID00027941 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00027942 | Nucleus | Colon adenocarcinoma cell | Microarray | 20864815 |
| RLID00027943 | Nucleus | HeLa cell | Next-generation sequencing | 22058130 |
| RLID00027944 | Circulating | Plasma | Next-generation sequencing | 25415674 |
| RLID00027945 | Nucleolus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027946 | Exosome | Serum | Next-generation sequencing | 24683445 |
| RLID00027947 | Exosome | Plasma | Next-generation sequencing | 24683445 |
| RLID00027948 | Circulating | Serum | qRT-PCR | 18766170 |
| RLID00027949 | Cytoplasm | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027950 | Nucleus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027951 | Endoplasmic reticulum | HEK293 cell | Northern blo|RT-PCR | 26304123 |
| RLID00027952 | Exosome | Gastric cancer cell | - | 22529849 |
| RLID00027953 | Exosome | Glioblastoma cell | - | 22529849 |
| RLID00027954 | Exosome | Renal carcinomas cell | - | 22529849 |
| RLID00027955 | Circulating | Serum | Next-generation sequencing | 22719975 |