Detail
| RLID: | RLID00027932 |
| RNA Symbol: | hsa-let-7a-5p |
| RNA Category: | miRNA |
| Organism: | Homo sapiens |
| Aliase: | hsa-let-7a |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Exosome | Breast cancer cell | RT-PCR|Microarray | 24468161 |
| Description: Figure 3: Differential gene expression in exosome-like vesicles. A) Hierarchical clustering was used to display miRNAs differentially expressed in each vesicle type. The extent of green (decreased fold change) or red (increased fold change) colors is directly proportional to the magnitude of differential expression of miRNAs. To perform these comparisons, probe sets whose target was not detected in any sample were eliminated from the data matrix. The data were grouped by type of exosome-like vesicles and members of each group were pooled, before a Student's t-test was used to identify those miRNAs that were expressed in a statistically significant manner (P < 0.05). B) miRNA profiles of the MDA-Exo versus MCF-Exo. Bars represent fold change of hybridization signals in MDA-Exo against MCF-Exo. Blue and red bars display relatively higher miRNA expressions in MDA-Exo and MCF-Exo, respectively. The miRNAs were quantified using the universal reference in equimolar concentrations and cross referenced with experimental data. Then the expression values were compared in two types of exosome-like vesicles (three independent experiments; P < 0.05). Data are collected from Figure 3. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00027918 | Circulating | Plasma | qRT-PCR | 22262318 |
| RLID00027919 | Circulating | Cerebrospinal fluid | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00027920 | Circulating | Serum | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00027921 | Circulating | Serum | Next-generation sequencing | 23641832 |
| RLID00027922 | Circulating | Plasma | qRT-PCR | 24937531 |
| RLID00027923 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00027924 | Cytoplasm | HeLa cell | Microarray | 21695135 |
| RLID00027925 | Exosome | Serum | Microarray | 18589210 |
| RLID00027926 | Exosome | Breast cancer cell | Microarray | 20976003 |
| RLID00027927 | Exosome | Dendritic cell | Microarray | 21505438 |
| RLID00027928 | Exosome | B cell | Microarray | 21505438 |
| RLID00027929 | Exosome | Serum | RT-PCR | 22427800 |
| RLID00027930 | Exosome | Brain tissue | Microarray | 23382797 |
| RLID00027931 | Exosome | Plasma | Next-generation sequencing | 23663360 |
| RLID00027933 | Exosome | Esophageal cancer cell | Next-generation sequencing | 25184951 |
| RLID00027934 | Microvesicle | Plasma | qRT-PCR | 19002258 |
| RLID00027935 | Microvesicle | Glioblastoma cell | qRT-PCR|Microarray | 19011622 |
| RLID00027936 | Microvesicle | Plasma | Next-generation sequencing | 23077538 |
| RLID00027937 | Microvesicle | Seminal Plasma | Microarray | 23539611 |
| RLID00027938 | Microvesicle | Hepatoma cell | qRT-PCR|Microarray | 23771658 |
| RLID00027939 | Mitochondrion | HeLa cell | Next-generation sequencing | 22984580 |
| RLID00027940 | Mitochondrion | Eembryonic kidney cell | Next-generation sequencing | 22984580 |
| RLID00027941 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00027942 | Nucleus | Colon adenocarcinoma cell | Microarray | 20864815 |
| RLID00027943 | Nucleus | HeLa cell | Next-generation sequencing | 22058130 |
| RLID00027944 | Circulating | Plasma | Next-generation sequencing | 25415674 |
| RLID00027945 | Nucleolus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027946 | Exosome | Serum | Next-generation sequencing | 24683445 |
| RLID00027947 | Exosome | Plasma | Next-generation sequencing | 24683445 |
| RLID00027948 | Circulating | Serum | qRT-PCR | 18766170 |
| RLID00027949 | Cytoplasm | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027950 | Nucleus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027951 | Endoplasmic reticulum | HEK293 cell | Northern blo|RT-PCR | 26304123 |
| RLID00027952 | Exosome | Gastric cancer cell | - | 22529849 |
| RLID00027953 | Exosome | Glioblastoma cell | - | 22529849 |
| RLID00027954 | Exosome | Renal carcinomas cell | - | 22529849 |
| RLID00027955 | Circulating | Serum | Next-generation sequencing | 22719975 |