Detail
| RLID: | RLID00027951 |
| RNA Symbol: | hsa-let-7a-5p |
| RNA Category: | miRNA |
| Organism: | Homo sapiens |
| Aliase: | hsa-let-7a |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Endoplasmic reticulum | HEK293 cell | Northern blo|RT-PCR | 26304123 |
| Description: To confirm the association of miRNA and Ago2 protein with rER and associated structures, we fractionated isotonic HEK293 cell lysates on a 3-30% OptiPrep gradient and analyzed the individual fractions. We observed that the majority of Ago2 and let-7a miRNA accumulated with the ER-positive fractions (Fig. 1C). | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00027918 | Circulating | Plasma | qRT-PCR | 22262318 |
| RLID00027919 | Circulating | Cerebrospinal fluid | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00027920 | Circulating | Serum | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00027921 | Circulating | Serum | Next-generation sequencing | 23641832 |
| RLID00027922 | Circulating | Plasma | qRT-PCR | 24937531 |
| RLID00027923 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00027924 | Cytoplasm | HeLa cell | Microarray | 21695135 |
| RLID00027925 | Exosome | Serum | Microarray | 18589210 |
| RLID00027926 | Exosome | Breast cancer cell | Microarray | 20976003 |
| RLID00027927 | Exosome | Dendritic cell | Microarray | 21505438 |
| RLID00027928 | Exosome | B cell | Microarray | 21505438 |
| RLID00027929 | Exosome | Serum | RT-PCR | 22427800 |
| RLID00027930 | Exosome | Brain tissue | Microarray | 23382797 |
| RLID00027931 | Exosome | Plasma | Next-generation sequencing | 23663360 |
| RLID00027932 | Exosome | Breast cancer cell | RT-PCR|Microarray | 24468161 |
| RLID00027933 | Exosome | Esophageal cancer cell | Next-generation sequencing | 25184951 |
| RLID00027934 | Microvesicle | Plasma | qRT-PCR | 19002258 |
| RLID00027935 | Microvesicle | Glioblastoma cell | qRT-PCR|Microarray | 19011622 |
| RLID00027936 | Microvesicle | Plasma | Next-generation sequencing | 23077538 |
| RLID00027937 | Microvesicle | Seminal Plasma | Microarray | 23539611 |
| RLID00027938 | Microvesicle | Hepatoma cell | qRT-PCR|Microarray | 23771658 |
| RLID00027939 | Mitochondrion | HeLa cell | Next-generation sequencing | 22984580 |
| RLID00027940 | Mitochondrion | Eembryonic kidney cell | Next-generation sequencing | 22984580 |
| RLID00027941 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00027942 | Nucleus | Colon adenocarcinoma cell | Microarray | 20864815 |
| RLID00027943 | Nucleus | HeLa cell | Next-generation sequencing | 22058130 |
| RLID00027944 | Circulating | Plasma | Next-generation sequencing | 25415674 |
| RLID00027945 | Nucleolus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027946 | Exosome | Serum | Next-generation sequencing | 24683445 |
| RLID00027947 | Exosome | Plasma | Next-generation sequencing | 24683445 |
| RLID00027948 | Circulating | Serum | qRT-PCR | 18766170 |
| RLID00027949 | Cytoplasm | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027950 | Nucleus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00027952 | Exosome | Gastric cancer cell | - | 22529849 |
| RLID00027953 | Exosome | Glioblastoma cell | - | 22529849 |
| RLID00027954 | Exosome | Renal carcinomas cell | - | 22529849 |
| RLID00027955 | Circulating | Serum | Next-generation sequencing | 22719975 |