Detail

RLID: RLID00028190
RNA Symbol: hsa-miR-16-5p
RNA Category: miRNA
Organism: Homo sapiens
Aliase: hsa-miR-16
Sequence:
Homology: bta-miR-15b (Bos taurus), bta-miR-16a (Bos taurus)
Network:
Subcellular Localization:
Localization Tissue Validated Method PMID
Exosome A549|HepG2 Microarrays|RT-PCR 20615901
Description: These observations of miRNA distribution demonstrate that the miRNA export system in the cells must somehow be able to target specific miRNAs to specific extracellular structures, but does not exclusively channel specific miRNAs to a given fraction. There are, however, some miRNAs that are found in the supernatant almost to the exclusion of all other fractions (miR-219 in A549 culture medium, Figure 4b, for example). Another significant observation from this data is that even though we showed that the two cell lines studied here export a similar spectrum of miRNAs (Figure 1b) the distributions of miRNAs from the two cell lines demonstrate clear differences, as shown in Figure 4b and c. For example, miR-145 is present at very low levels in HepG2 derived microvesicles (Figure 4c), while the same miRNA is present at significant levels in both A549 derived microvesicles and exosomes (Figure 4b). On the other hand, there are some miRNAs, notably those that are almost uniformly distributed among the fractions, with the same distribution no matter which cell-line is the source. These observations certainly indicate the degree of complexity of this export system that will bear further investigation. Data are collected from Figure 4.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00028159 Circulating Serum Microarray 19597549
RLID00028160 Circulating Plasma qRT-PCR|Microarray 20883153
RLID00028161 Circulating Plasma qRT-PCR 22262318
RLID00028162 Circulating Cerebrospinal fluid Next-generation sequencing|RT-PCR 23525801
RLID00028163 Circulating Serum Next-generation sequencing|RT-PCR 23525801
RLID00028164 Circulating Serum Next-generation sequencing 23641832
RLID00028165 Circulating Follicular fluid Microarray 23666971
RLID00028166 Cytoplasm Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028167 Cytoplasm HeLa cell Microarray 21695135
RLID00028168 Exosome Serum Microarray 18589210
RLID00028169 Exosome Dendritic cell Microarray 21505438
RLID00028170 Exosome B cell Microarray 21505438
RLID00028171 Exosome Brain tissue Microarray 23382797
RLID00028172 Exosome Plasma Next-generation sequencing 23663360
RLID00028173 Exosome Breast cancer cell Microarray 24468161
RLID00028174 Microvesicle Plasma qRT-PCR 19002258
RLID00028175 Microvesicle Glioblastoma cell qRT-PCR|Microarray 19011622
RLID00028176 Microvesicle Plasma Next-generation sequencing 23077538
RLID00028177 Microvesicle Seminal Plasma Microarray 23539611
RLID00028178 Microvesicle Hepatoma cell qRT-PCR|Microarray 23771658
RLID00028179 Mitochondrion HeLa cell Next-generation sequencing 22984580
RLID00028180 Mitochondrion Eembryonic kidney cell Next-generation sequencing 22984580
RLID00028181 Nucleus Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028182 Nucleus Neural progenitor cell qRT-PCR|Microarray 21363885
RLID00028183 Nucleolus HeLa|MCF7 Microarray 24918059
RLID00028184 Exosome Serum Next-generation sequencing 24683445
RLID00028185 Exosome Plasma Next-generation sequencing 24683445
RLID00028186 Cytoplasm HeLa|MCF7 Microarray 24918059
RLID00028187 Nucleus HeLa|MCF7 Microarray 24918059
RLID00028188 Circulating Plasma RT-PCR 24633190
RLID00028189 Microvesicle A549|HepG2 Microarrays|RT-PCR 20615901
RLID00028191 Exosome Epithelial cell - 22529849
RLID00028192 Exosome Liver carcinoma cell - 22529849
RLID00028193 Exosome Kidney cell - 22529849
RLID00028194 Exosome Glioblastoma cell - 22529849
RLID00028195 Circulating Serum Next-generation sequencing 22719975
RLID00028196 Circulating Plasma RT-PCR 24130905