Detail
| RLID: | RLID00028331 |
| RNA Symbol: | hsa-miR-20a-5p |
| RNA Category: | miRNA |
| Organism: | Homo sapiens |
| Aliase: | hsa-miR-20, hsa-miR-20a |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Circulating | Blood | RT-PCR|Microarray | 20145944 |
| Description: Our results highlight four miRNA markers for blood identification (miR-20a, miR-106a, miR-185, and miR-144) and five for semen identification (miR-135a, miR-10a, miR-507, miR-943, and miR-891a). Of those, two miRNA markers for blood (miR-144 and miR-185) and two others for semen (miR-135a and miR-897a) are suggestive to be most useful for body fluid identification in future forensic applications, and the respective RT-PCR assays used here for their detection were highly sensitive, allowing the reliable marker detection from subpicogram amounts of total RNA. Our results proved the applicability of the miRNA approach for forensic body fluids identification. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00028332 | Circulating | Plasma | qRT-PCR | 22262318 |
| RLID00028333 | Circulating | Serum | qRT-PCR | 22798503 |
| RLID00028334 | Circulating | Serum | qRT-PCR | 23056289 |
| RLID00028335 | Circulating | Cerebrospinal fluid | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00028336 | Circulating | Serum | - | 24456939 |
| RLID00028337 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00028338 | Cytoplasm | Neural progenitor cell | qRT-PCR|Microarray | 21363885 |
| RLID00028339 | Cytoplasm | HeLa cell | Microarray | 21695135 |
| RLID00028340 | Extracellular vesicle | HEK293T|HMEC-1|Mononuclear cell | Next-generation sequencing | 22849433 |
| RLID00028341 | Exosome | Serum | Microarray | 18589210 |
| RLID00028342 | Exosome | Dendritic cell | Microarray | 21505438 |
| RLID00028343 | Exosome | B cell | Microarray | 21505438 |
| RLID00028344 | Exosome | Breast milk | Next-generation sequencing | 22211110 |
| RLID00028345 | Exosome | Malignant melanoma cell | Microarray | 23056502 |
| RLID00028346 | Exosome | Brain tissue | Microarray | 23382797 |
| RLID00028347 | Exosome | Plasma | Next-generation sequencing | 23663360 |
| RLID00028348 | Exosome | Serum | Next-generation sequencing | 25349172 |
| RLID00028349 | Microvesicle | Plasma | qRT-PCR | 19002258 |
| RLID00028350 | Microvesicle | Glioblastoma cell | qRT-PCR|Microarray | 19011622 |
| RLID00028351 | Microvesicle | Seminal Plasma | Microarray | 23539611 |
| RLID00028352 | Microvesicle | Follicular fluid | Microarray | 23666971 |
| RLID00028353 | Microvesicle | Hepatoma cell | qRT-PCR|Microarray | 23771658 |
| RLID00028354 | Mitochondrion | Skeletal muscular cell | In situ hybridization|qRT-PCR | 21637849 |
| RLID00028355 | Mitochondrion | HeLa cell | Next-generation sequencing | 22984580 |
| RLID00028356 | Mitochondrion | Eembryonic kidney cell | Next-generation sequencing | 22984580 |
| RLID00028357 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00028358 | Nucleus | Colon adenocarcinoma cell | Microarray | 20864815 |
| RLID00028359 | Nucleus | HeLa cell | Next-generation sequencing | 22058130 |
| RLID00028360 | Exosome | Cerebrospinal fluid | Next-generation sequencing | 25420022 |
| RLID00028361 | Circulating | Plasma | Next-generation sequencing | 25415674 |
| RLID00028362 | Nucleolus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00028363 | Exosome | Serum | Next-generation sequencing | 24683445 |
| RLID00028364 | Exosome | Plasma | Next-generation sequencing | 24683445 |
| RLID00028365 | Cytoplasm | HeLa|MCF7 | Microarray | 24918059 |
| RLID00028366 | Nucleus | HeLa|MCF7 | Microarray | 24918059 |
| RLID00028367 | Cytoplasm | HeLa cell | qRT-PCR|In situ hybridization | 23940654 |
| RLID00028368 | Exosome | Glioblastoma cell | - | 22529849 |
| RLID00028369 | Circulating | Serum | Next-generation sequencing | 22719975 |