Detail

RLID: RLID00028446
RNA Symbol: hsa-miR-22-3p
RNA Category: miRNA
Organism: Homo sapiens
Aliase: hsa-miR-22
Sequence:
Homology: eca-miR-22 (Equus caballus), hsa-miR-22-5p (Homo sapiens)
Network:
Subcellular Localization:
Localization Tissue Validated Method PMID
Microvesicle A549|HepG2 Microarrays|RT-PCR 20615901
Description: These observations of miRNA distribution demonstrate that the miRNA export system in the cells must somehow be able to target specific miRNAs to specific extracellular structures, but does not exclusively channel specific miRNAs to a given fraction. There are, however, some miRNAs that are found in the supernatant almost to the exclusion of all other fractions (miR-219 in A549 culture medium, Figure 4b, for example). Another significant observation from this data is that even though we showed that the two cell lines studied here export a similar spectrum of miRNAs (Figure 1b) the distributions of miRNAs from the two cell lines demonstrate clear differences, as shown in Figure 4b and c. For example, miR-145 is present at very low levels in HepG2 derived microvesicles (Figure 4c), while the same miRNA is present at significant levels in both A549 derived microvesicles and exosomes (Figure 4b). On the other hand, there are some miRNAs, notably those that are almost uniformly distributed among the fractions, with the same distribution no matter which cell-line is the source. These observations certainly indicate the degree of complexity of this export system that will bear further investigation. Data are collected from Figure 4.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00028420 Circulating Cerebrospinal fluid Next-generation sequencing|RT-PCR 23525801
RLID00028421 Circulating Serum Next-generation sequencing|RT-PCR 23525801
RLID00028422 Circulating Serum Next-generation sequencing 23641832
RLID00028423 Circulating Serum Next-generation sequencing 25359176
RLID00028424 Cytoplasm Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028425 Extracellular vesicle HEK293T|HMEC-1|Mononuclear cell Next-generation sequencing 22849433
RLID00028426 Exosome Serum Microarray 18589210
RLID00028427 Exosome Dendritic cell Microarray 21505438
RLID00028428 Exosome B cell Microarray 21505438
RLID00028429 Exosome Malignant melanoma cell Microarray 23056502
RLID00028430 Exosome Brain tissue Microarray 23382797
RLID00028431 Exosome Plasma Next-generation sequencing 23663360
RLID00028432 Microvesicle Plasma Next-generation sequencing 23077538
RLID00028433 Microvesicle Seminal Plasma Microarray 23539611
RLID00028434 Microvesicle Hepatoma cell qRT-PCR|Microarray 23771658
RLID00028435 Mitochondrion Skeletal muscular cell In situ hybridization|qRT-PCR 21637849
RLID00028436 Mitochondrion HeLa cell Next-generation sequencing 22984580
RLID00028437 Mitochondrion Eembryonic kidney cell Next-generation sequencing 22984580
RLID00028438 Nucleus Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028439 Nucleus HeLa cell Next-generation sequencing 22058130
RLID00028440 Exosome Cerebrospinal fluid Next-generation sequencing 25420022
RLID00028441 Circulating Plasma Next-generation sequencing 25415674
RLID00028442 Nucleolus HeLa|MCF7 Microarray 24918059
RLID00028443 Exosome Serum Next-generation sequencing 24683445
RLID00028444 Exosome Plasma Next-generation sequencing 24683445
RLID00028445 Nucleus HeLa|MCF7 Microarray 24918059
RLID00028447 Exosome A549|HepG2 Microarrays|RT-PCR 20615901
RLID00028448 Exosome Ovarian - 22529849
RLID00028449 Exosome Renal carcinomas cell - 22529849
RLID00028450 Exosome Epithelial cell - 22529849
RLID00028451 Exosome Liver carcinoma cell - 22529849
RLID00028452 Circulating Serum Next-generation sequencing 22719975
RLID00028453 Exosome Mast cell Microarray 24009880