Detail

RLID: RLID00028487
RNA Symbol: hsa-miR-23a-3p
RNA Category: miRNA
Organism: Homo sapiens
Aliase: hsa-miR-23a
Sequence:
Homology: bta-miR-23a (Bos taurus), eca-miR-23a (Equus caballus)
Network:
Subcellular Localization:
Localization Tissue Validated Method PMID
Exosome A549|HepG2 Microarrays|RT-PCR 20615901
Description: These observations of miRNA distribution demonstrate that the miRNA export system in the cells must somehow be able to target specific miRNAs to specific extracellular structures, but does not exclusively channel specific miRNAs to a given fraction. There are, however, some miRNAs that are found in the supernatant almost to the exclusion of all other fractions (miR-219 in A549 culture medium, Figure 4b, for example). Another significant observation from this data is that even though we showed that the two cell lines studied here export a similar spectrum of miRNAs (Figure 1b) the distributions of miRNAs from the two cell lines demonstrate clear differences, as shown in Figure 4b and c. For example, miR-145 is present at very low levels in HepG2 derived microvesicles (Figure 4c), while the same miRNA is present at significant levels in both A549 derived microvesicles and exosomes (Figure 4b). On the other hand, there are some miRNAs, notably those that are almost uniformly distributed among the fractions, with the same distribution no matter which cell-line is the source. These observations certainly indicate the degree of complexity of this export system that will bear further investigation. Data are collected from Figure 4.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00028454 Circulating Serum qRT-PCR 21098710
RLID00028455 Circulating Cerebrospinal fluid Next-generation sequencing|RT-PCR 23525801
RLID00028456 Circulating Serum Next-generation sequencing|RT-PCR 23525801
RLID00028457 Circulating Serum Next-generation sequencing 23641832
RLID00028458 Circulating Serum qRT-PCR 24981880
RLID00028459 Circulating Serum Next-generation sequencing 25359176
RLID00028460 Cytoplasm Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028461 Cytoplasm HeLa cell Microarray 21695135
RLID00028462 Extracellular vesicle HEK293T|HMEC-1|Mononuclear cell Next-generation sequencing 22849433
RLID00028463 Exosome Serum Microarray 18589210
RLID00028464 Exosome Breast cancer cell Microarray 20976003
RLID00028465 Exosome Dendritic cell Microarray 21505438
RLID00028466 Exosome B cell Microarray 21505438
RLID00028467 Exosome Malignant melanoma cell Microarray 23056502
RLID00028468 Exosome Brain tissue Microarray 23382797
RLID00028469 Exosome Plasma Next-generation sequencing 23663360
RLID00028470 Microvesicle Plasma Next-generation sequencing 23077538
RLID00028471 Microvesicle Seminal Plasma Microarray 23539611
RLID00028472 Mitochondrion Skeletal muscular cell In situ hybridization|qRT-PCR 21637849
RLID00028473 Mitochondrion HeLa cell Next-generation sequencing 22984580
RLID00028474 Mitochondrion Eembryonic kidney cell Next-generation sequencing 22984580
RLID00028475 Nucleus Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028476 Nucleus Colon adenocarcinoma cell Microarray 20864815
RLID00028477 Nucleus HeLa cell Next-generation sequencing 22058130
RLID00028478 Circulating Plasma Next-generation sequencing 25415674
RLID00028479 Nucleolus HeLa|MCF7 Microarray 24918059
RLID00028480 Exosome HeLa cell Next-generation sequencing 24205503
RLID00028481 Exosome Colon Cancer Cell Line qRT-PCR|RNA-seq 25330373
RLID00028482 Exosome Serum Next-generation sequencing 24683445
RLID00028483 Exosome Plasma Next-generation sequencing 24683445
RLID00028484 Cytoplasm HeLa|MCF7 Microarray 24918059
RLID00028485 Nucleus HeLa|MCF7 Microarray 24918059
RLID00028486 Microvesicle A549|HepG2 Microarrays|RT-PCR 20615901
RLID00028488 Exosome Ovarian - 22529849
RLID00028489 Exosome Renal carcinomas cell - 22529849
RLID00028490 Exosome Epithelial cell - 22529849
RLID00028491 Exosome Liver carcinoma cell - 22529849
RLID00028492 Circulating Serum Next-generation sequencing 22719975
RLID00028493 Exosome Mast cell Microarray 24009880