Detail

RLID: RLID00028619
RNA Symbol: hsa-miR-26b-5p
RNA Category: miRNA
Organism: Homo sapiens
Aliase: hsa-miR-26b
Sequence:
Homology: hsa-miR-26a-1-3p (Homo sapiens), hsa-miR-26a-2-3p (Homo sapiens)
Network:
Subcellular Localization:
Localization Tissue Validated Method PMID
Microvesicle A549|HepG2 Microarrays|RT-PCR 20615901
Description: These observations of miRNA distribution demonstrate that the miRNA export system in the cells must somehow be able to target specific miRNAs to specific extracellular structures, but does not exclusively channel specific miRNAs to a given fraction. There are, however, some miRNAs that are found in the supernatant almost to the exclusion of all other fractions (miR-219 in A549 culture medium, Figure 4b, for example). Another significant observation from this data is that even though we showed that the two cell lines studied here export a similar spectrum of miRNAs (Figure 1b) the distributions of miRNAs from the two cell lines demonstrate clear differences, as shown in Figure 4b and c. For example, miR-145 is present at very low levels in HepG2 derived microvesicles (Figure 4c), while the same miRNA is present at significant levels in both A549 derived microvesicles and exosomes (Figure 4b). On the other hand, there are some miRNAs, notably those that are almost uniformly distributed among the fractions, with the same distribution no matter which cell-line is the source. These observations certainly indicate the degree of complexity of this export system that will bear further investigation. Data are collected from Figure 4.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00028594 Circulating Cerebrospinal fluid Next-generation sequencing|RT-PCR 23525801
RLID00028595 Circulating Serum Next-generation sequencing 23641832
RLID00028596 Circulating Follicular fluid Microarray 23666971
RLID00028597 Cytoplasm Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028598 Extracellular vesicle HEK293T|HMEC-1|Mononuclear cell Next-generation sequencing 22849433
RLID00028599 Exosome Serum Microarray 18589210
RLID00028600 Exosome Breast cancer cell Microarray 20976003
RLID00028601 Exosome Dendritic cell Microarray 21505438
RLID00028602 Exosome B cell Microarray 21505438
RLID00028603 Exosome Breast milk Next-generation sequencing 22211110
RLID00028604 Exosome Epidermal melanocyte Microarray 23056502
RLID00028605 Exosome Brain tissue Microarray 23382797
RLID00028606 Exosome Plasma Next-generation sequencing 23663360
RLID00028607 Microvesicle Plasma qRT-PCR 19002258
RLID00028608 Microvesicle Plasma Next-generation sequencing 23077538
RLID00028609 Microvesicle Seminal Plasma Microarray 23539611
RLID00028610 Mitochondrion HeLa cell Next-generation sequencing 22984580
RLID00028611 Mitochondrion Eembryonic kidney cell Next-generation sequencing 22984580
RLID00028612 Nucleus Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028613 Nucleus Colon adenocarcinoma cell Microarray 20864815
RLID00028614 Nucleus HeLa cell Next-generation sequencing 22058130
RLID00028615 Exosome Serum Next-generation sequencing 24683445
RLID00028616 Exosome Plasma Next-generation sequencing 24683445
RLID00028617 Cytoplasm HeLa|MCF7 Microarray 24918059
RLID00028618 Nucleus HeLa|MCF7 Microarray 24918059
RLID00028620 Exosome A549|HepG2 Microarrays|RT-PCR 20615901
RLID00028621 Exosome Renal carcinomas cell - 22529849
RLID00028622 Exosome Liver carcinoma cell - 22529849
RLID00028623 Circulating Serum Next-generation sequencing 22719975