Detail

RLID: RLID00028705
RNA Symbol: hsa-miR-29a-3p
RNA Category: miRNA
Organism: Homo sapiens
Aliase: hsa-miR-29a
Sequence:
Homology: aae-miR-285 (Aedes aegypti), bta-miR-29a (Bos taurus)
Network:
Subcellular Localization:
Localization Tissue Validated Method PMID
Exosome A549|HepG2 Microarrays|RT-PCR 20615901
Description: These observations of miRNA distribution demonstrate that the miRNA export system in the cells must somehow be able to target specific miRNAs to specific extracellular structures, but does not exclusively channel specific miRNAs to a given fraction. There are, however, some miRNAs that are found in the supernatant almost to the exclusion of all other fractions (miR-219 in A549 culture medium, Figure 4b, for example). Another significant observation from this data is that even though we showed that the two cell lines studied here export a similar spectrum of miRNAs (Figure 1b) the distributions of miRNAs from the two cell lines demonstrate clear differences, as shown in Figure 4b and c. For example, miR-145 is present at very low levels in HepG2 derived microvesicles (Figure 4c), while the same miRNA is present at significant levels in both A549 derived microvesicles and exosomes (Figure 4b). On the other hand, there are some miRNAs, notably those that are almost uniformly distributed among the fractions, with the same distribution no matter which cell-line is the source. These observations certainly indicate the degree of complexity of this export system that will bear further investigation. Data are collected from Figure 4.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00028676 Circulating Serum qRT-PCR 18954897
RLID00028677 Circulating Cerebrospinal fluid Next-generation sequencing|RT-PCR 23525801
RLID00028678 Circulating Serum Next-generation sequencing|RT-PCR 23525801
RLID00028679 Circulating Serum Next-generation sequencing 23641832
RLID00028680 Circulating Follicular fluid Microarray 23666971
RLID00028681 Cytoplasm Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028682 Cytoplasm HeLa cell Microarray 21695135
RLID00028683 Exosome Serum Microarray 18589210
RLID00028684 Exosome Dendritic cell Microarray 21505438
RLID00028685 Exosome B cell Microarray 21505438
RLID00028686 Exosome Malignant melanoma cell Microarray 23056502
RLID00028687 Exosome Brain tissue Microarray 23382797
RLID00028688 Exosome Plasma Next-generation sequencing 23663360
RLID00028689 Microvesicle Plasma qRT-PCR|Microarray 19951903
RLID00028690 Microvesicle Plasma Next-generation sequencing 23077538
RLID00028691 Microvesicle Seminal Plasma Microarray 23539611
RLID00028692 Microvesicle Follicular fluid Microarray 23666971
RLID00028693 Microvesicle Hepatoma cell qRT-PCR|Microarray 23771658
RLID00028694 Mitochondrion Skeletal muscular cell In situ hybridization|qRT-PCR 21637849
RLID00028695 Mitochondrion HeLa cell Next-generation sequencing 22984580
RLID00028696 Mitochondrion Eembryonic kidney cell Next-generation sequencing 22984580
RLID00028697 Nucleus Nasopharyngeal carcinoma cell Next-generation sequencing 20498841
RLID00028698 Nucleus HeLa cell Next-generation sequencing 22058130
RLID00028699 Circulating Plasma Next-generation sequencing 25415674
RLID00028700 Nucleolus HeLa|MCF7 Microarray 24918059
RLID00028701 Exosome Serum Next-generation sequencing 24683445
RLID00028702 Exosome Plasma Next-generation sequencing 24683445
RLID00028703 Nucleus HeLa|MCF7 Microarray 24918059
RLID00028704 Microvesicle A549|HepG2 Microarrays|RT-PCR 20615901
RLID00028706 Exosome Renal carcinomas cell - 22529849
RLID00028707 Exosome Epithelial cell - 22529849
RLID00028708 Exosome Liver carcinoma cell - 22529849
RLID00028709 Circulating Serum Next-generation sequencing 22719975
RLID00028710 Exosome Mast cell Microarray 24009880