Detail

RLID: RLID00032586
RNA Symbol: hsa-miR-451a
RNA Category: miRNA
Organism: Homo sapiens
Aliase: hsa-miR-451
Sequence:
Homology: hsa-miR-451b (Homo sapiens), hsa-mir-451a (Homo sapiens)
Network:
Subcellular Localization:
Localization Tissue Validated Method PMID
Microvesicle A549|HepG2 Microarrays|RT-PCR 20615901
Description: These observations of miRNA distribution demonstrate that the miRNA export system in the cells must somehow be able to target specific miRNAs to specific extracellular structures, but does not exclusively channel specific miRNAs to a given fraction. There are, however, some miRNAs that are found in the supernatant almost to the exclusion of all other fractions (miR-219 in A549 culture medium, Figure 4b, for example). Another significant observation from this data is that even though we showed that the two cell lines studied here export a similar spectrum of miRNAs (Figure 1b) the distributions of miRNAs from the two cell lines demonstrate clear differences, as shown in Figure 4b and c. For example, miR-145 is present at very low levels in HepG2 derived microvesicles (Figure 4c), while the same miRNA is present at significant levels in both A549 derived microvesicles and exosomes (Figure 4b). On the other hand, there are some miRNAs, notably those that are almost uniformly distributed among the fractions, with the same distribution no matter which cell-line is the source. These observations certainly indicate the degree of complexity of this export system that will bear further investigation. Data are collected from Figure 4.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00032567 Exosome Breast milk Next-generation sequencing 22211110
RLID00032568 Circulating Plasma qRT-PCR 22262318
RLID00032569 Circulating Cerebrospinal fluid Next-generation sequencing|RT-PCR 23525801
RLID00032570 Circulating Serum Next-generation sequencing|RT-PCR 23525801
RLID00032571 Circulating Serum Next-generation sequencing 23641832
RLID00032572 Extracellular vesicle HEK293T|HMEC-1|Mononuclear cell Next-generation sequencing 22849433
RLID00032573 Exosome Breast cancer cell Microarray 20976003
RLID00032574 Exosome T cell Microarray 21505438
RLID00032575 Exosome B cell Microarray 21505438
RLID00032576 Circulating Serum Microarray 23169280
RLID00032577 Exosome Brain tissue Microarray 23382797
RLID00032578 Exosome Plasma Next-generation sequencing 23663360
RLID00032579 Microvesicle Plasma Next-generation sequencing 23077538
RLID00032580 Extracellular vesicle Colon Cancer Cell Line qRT-PCR|RNA-seq 25330373
RLID00032581 Exosome B cell Next-generation sequencing 25242326
RLID00032582 Exosome Serum Next-generation sequencing 24683445
RLID00032583 Exosome Plasma Next-generation sequencing 24683445
RLID00032584 Circulating Serum qRT-PCR 18766170
RLID00032585 Circulating Plasma RT-PCR 24633190
RLID00032587 Exosome A549|HepG2 Microarrays|RT-PCR 20615901
RLID00032588 Exosome HCC cell - 22529849
RLID00032589 Exosome Renal carcinomas cell - 22529849
RLID00032590 Circulating Serum Next-generation sequencing 22719975
RLID00032591 Exosome Mast cell Microarray 24009880
RLID00032592 Circulating Plasma RT-PCR 24130905
RLID00032593 Microvesicle Liver stem cell qRT-PCR 20668554
RLID00032594 Microvesicle MSC cell qRT-PCR 20668554