Detail
| RLID: | RLID00033640 |
| RNA Symbol: | hsa-miR-421 |
| RNA Category: | miRNA |
| Organism: | Homo sapiens |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Microvesicle | Seminal Plasma | Microarray | 23539611 |
| Description: MiRNAs shuttled by SMVs in seminal plasma are potent non-invasive biomarkers to evaluate defects originating from the different organs of the male reproductive tract, including the epididymis (Wang et al., 2011). In order to assess the reversibility of post-vasectomy miRNA sequelae, we investigated whether SMV miRNAs altered by vasectomy could be retrieved in SMVs from normospermic vasovasostomized donors. miRNA microarray profiling performed on SMVs from normal, vasectomized and vasovasostomized donors identified 313 miRNAs whose intensity was above the threshold of detection. Among these miRNAs, 293 were detected in samples from normal donors, 275 in vasectomized donors and 298 in vasovasostomized donors (Fig. 3, Supplementary data, Table SIV). Data are collected from Table S4. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00033633 | Circulating | Cerebrospinal fluid | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00033634 | Circulating | Serum | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00033635 | Circulating | Gastric juice | - | 24456939 |
| RLID00033636 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00033637 | Extracellular vesicle | HEK293T|HMEC-1|Mononuclear cell | Next-generation sequencing | 22849433 |
| RLID00033638 | Exosome | Serum | Microarray | 18589210 |
| RLID00033639 | Exosome | Plasma | Next-generation sequencing | 23663360 |
| RLID00033641 | Mitochondrion | Skeletal muscular cell | In situ hybridization|qRT-PCR | 21637849 |
| RLID00033642 | Mitochondrion | HeLa cell | Next-generation sequencing | 22984580 |
| RLID00033643 | Mitochondrion | Eembryonic kidney cell | Next-generation sequencing | 22984580 |
| RLID00033644 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00033645 | Nucleus | HeLa cell | Next-generation sequencing | 22058130 |
| RLID00033646 | Exosome | Serum | Next-generation sequencing | 24683445 |
| RLID00033647 | Exosome | Plasma | Next-generation sequencing | 24683445 |
| RLID00033648 | Nucleus | HeLa|MCF7 | Microarray | 24918059 |