Detail
| RLID: | RLID00034623 |
| RNA Symbol: | hsa-miR-423-5p |
| RNA Category: | miRNA |
| Organism: | Homo sapiens |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Exosome | Plasma | qRT-PCR | 24937531 |
| Description: For miRNome analysis, two homogeneous samples were created: one by pooling 2 æ¸L of RNA from exosomes of diabetic patients and the other by pooling RNA from exosomes of control subjects. After reverse transcription (mirCURY LNA universal cDNA synthesis kit; Exiqon), the reaction was performed on a serum/plasma focus miRNA PCR panel 384 well (V1.R) (Exiqon) using the ABI 7900HT (Applied Biosystems). The most stable miRNAs (miR-425 and miR-423-5p) were identified by NormFinder and GeNorm, validated on each sample, and used as normalizators for circulating miRNAs. - See more at: http://press.endocrine.org/doi/10.1210/jc.2013-3843?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmedsthash.kodyGYkk.dpuf | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00034601 | Circulating | Cerebrospinal fluid | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00034602 | Circulating | Serum | Next-generation sequencing|RT-PCR | 23525801 |
| RLID00034603 | Circulating | Serum | Next-generation sequencing | 23641832 |
| RLID00034604 | Circulating | Follicular fluid | Microarray | 23666971 |
| RLID00034605 | Circulating | Serum | - | 24456939 |
| RLID00034606 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00034607 | Exosome | B cell | Microarray | 21505438 |
| RLID00034608 | Exosome | Brain tissue | Microarray | 23382797 |
| RLID00034609 | Exosome | K562 cell | Microarray | 24460325 |
| RLID00034610 | Exosome | Esophageal cancer cell | Next-generation sequencing | 25184951 |
| RLID00034611 | Microvesicle | Plasma | Next-generation sequencing | 23077538 |
| RLID00034612 | Microvesicle | Follicular fluid | Microarray | 23666971 |
| RLID00034613 | Mitochondrion | Skeletal muscular cell | In situ hybridization|qRT-PCR | 21637849 |
| RLID00034614 | Mitochondrion | HeLa cell | Next-generation sequencing | 22984580 |
| RLID00034615 | Mitochondrion | Eembryonic kidney cell | Next-generation sequencing | 22984580 |
| RLID00034616 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00034617 | Nucleus | HeLa cell | Next-generation sequencing | 22058130 |
| RLID00034618 | Circulating | Plasma | Next-generation sequencing | 25415674 |
| RLID00034619 | Exosome | Colon Cancer Cell Line | qRT-PCR|RNA-seq | 25330373 |
| RLID00034620 | Circulating | serum | Next-generation sequencing | 25323050 |
| RLID00034621 | Exosome | Serum | Next-generation sequencing | 24683445 |
| RLID00034622 | Exosome | Plasma | Next-generation sequencing | 24683445 |
| RLID00034624 | Circulating | Serum | Next-generation sequencing | 22719975 |
| RLID00034625 | Exosome | Mast cell | Microarray | 24009880 |