Detail
RLID: | RLID00037036 |
RNA Symbol: | Xlsirt |
RNA Category: | lncRNA |
Organism: | Xenopus laevis |
Subcellular Localization: |
Localization | Tissue | Validated Method | PMID |
Mitochondrion | Oocyte | In situ hybridization | 8948579 |
Description: In the present study we analyzed the properties of the METRO pathway, which localizes Xlsirt, Xcat2, and Xwnt11 RNAs to a specific region of the vegetal cortex during stage 1 of oogenesis. A combination of methodologies involving both fixed material and living oocytes was used to analyze RNA localization. We show that in early diplotene pre-stage 1 oocytes (25-50 microm in diameter) both endogenous and injected exogenous METRO RNAs translocated to multiple mitochondrial aggregates (pre-mitochondrial clouds) that surround the germinal vesicle (GV). However, by early stage 1 (diplotene oocytes, 50-200 microm), all three of the RNAs discriminated between the different clouds and translocated exclusively within the METRO of a single mitochondrial cloud. |
Other Subcellular Localization:
RLID | Localization | Tissue | Validated Method | PMID |
RLID00037032 | Mitochondrion | Oocyte | - | 7505061 |
RLID00037033 | Vegetal | Oocyte | - | 7505061 |
RLID00037034 | Mitochondrion | Oocyte | In situ hybridization | 7539356 |
RLID00037035 | Cytoplasm | Oocyte | In situ hybridization | 8948579 |
RLID00037037 | Vegetal | Oocyte | In situ hybridization | 8948579 |
RLID00037038 | Mitochondrion | Oocyte | In situ hybridization | 9545550 |
RLID00037039 | Endoplasmic reticulum | Oocyte | In situ hybridization | 9545550 |
RLID00037040 | Vegetal | Oocyte | In situ hybridization | 9545550 |
RLID00037041 | Vegetal | Oocyte | In situ hybridization | 9739112 |
RLID00037042 | Mitochondrion | Oocyte | In situ hybridization | 9739112 |
RLID00037043 | Germ plasm | Oocyte | In situ hybridization | 9739112 |
RLID00037044 | Germ plasm | Oocyte | In situ hybridization | 11784096 |
RLID00037045 | Mitochondrion | Oocyte | In situ hybridization | 11784096 |
RLID00037046 | Vegetal | Oocyte | In situ hybridization | 12676327 |
RLID00037047 | Mitochondrion | Oocyte | Quantitative RNA localization assay | 19223445 |