Detail

RLID: RLID00037040
RNA Symbol: Xlsirt
RNA Category: lncRNA
Organism: Xenopus laevis
Subcellular Localization:
Localization Tissue Validated Method PMID
Vegetal Oocyte In situ hybridization 9545550
Description: To definitively demonstrate the co-localization of Vg1 mRNA with the wedge-shaped ER structure we performed in situ hybridization to identify the location of the Vg1 mRNA, and immunostaining with GRP-78 to identify the position of the ER. During stage 1, while Vg1 mRNA was dispersed throughout the cytoplasm, very little ER was associated with the cortex. (D) Stage 3 oocyte showing endogenous Xlsirts (in situ hybridization) localized at the vegetal cortex (blue) at the leading edge of the wedge-shaped ER (red) (the arrow is pointing to the Xlsirts at the leading edge of the ER). Note that by stage 2 the ER is also uniformly distributed throughout the entire cortical region. (E) Late stage 3 oocyte showing Xlsirts localized within the cortex (blue) and the remnants of the wedge containing ER (red).

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00037032 Mitochondrion Oocyte - 7505061
RLID00037033 Vegetal Oocyte - 7505061
RLID00037034 Mitochondrion Oocyte In situ hybridization 7539356
RLID00037035 Cytoplasm Oocyte In situ hybridization 8948579
RLID00037036 Mitochondrion Oocyte In situ hybridization 8948579
RLID00037037 Vegetal Oocyte In situ hybridization 8948579
RLID00037038 Mitochondrion Oocyte In situ hybridization 9545550
RLID00037039 Endoplasmic reticulum Oocyte In situ hybridization 9545550
RLID00037041 Vegetal Oocyte In situ hybridization 9739112
RLID00037042 Mitochondrion Oocyte In situ hybridization 9739112
RLID00037043 Germ plasm Oocyte In situ hybridization 9739112
RLID00037044 Germ plasm Oocyte In situ hybridization 11784096
RLID00037045 Mitochondrion Oocyte In situ hybridization 11784096
RLID00037046 Vegetal Oocyte In situ hybridization 12676327
RLID00037047 Mitochondrion Oocyte Quantitative RNA localization assay 19223445