Detail
RLID: | RLID00037040 |
RNA Symbol: | Xlsirt |
RNA Category: | lncRNA |
Organism: | Xenopus laevis |
Subcellular Localization: |
Localization | Tissue | Validated Method | PMID |
Vegetal | Oocyte | In situ hybridization | 9545550 |
Description: To definitively demonstrate the co-localization of Vg1 mRNA with the wedge-shaped ER structure we performed in situ hybridization to identify the location of the Vg1 mRNA, and immunostaining with GRP-78 to identify the position of the ER. During stage 1, while Vg1 mRNA was dispersed throughout the cytoplasm, very little ER was associated with the cortex. (D) Stage 3 oocyte showing endogenous Xlsirts (in situ hybridization) localized at the vegetal cortex (blue) at the leading edge of the wedge-shaped ER (red) (the arrow is pointing to the Xlsirts at the leading edge of the ER). Note that by stage 2 the ER is also uniformly distributed throughout the entire cortical region. (E) Late stage 3 oocyte showing Xlsirts localized within the cortex (blue) and the remnants of the wedge containing ER (red). |
Other Subcellular Localization:
RLID | Localization | Tissue | Validated Method | PMID |
RLID00037032 | Mitochondrion | Oocyte | - | 7505061 |
RLID00037033 | Vegetal | Oocyte | - | 7505061 |
RLID00037034 | Mitochondrion | Oocyte | In situ hybridization | 7539356 |
RLID00037035 | Cytoplasm | Oocyte | In situ hybridization | 8948579 |
RLID00037036 | Mitochondrion | Oocyte | In situ hybridization | 8948579 |
RLID00037037 | Vegetal | Oocyte | In situ hybridization | 8948579 |
RLID00037038 | Mitochondrion | Oocyte | In situ hybridization | 9545550 |
RLID00037039 | Endoplasmic reticulum | Oocyte | In situ hybridization | 9545550 |
RLID00037041 | Vegetal | Oocyte | In situ hybridization | 9739112 |
RLID00037042 | Mitochondrion | Oocyte | In situ hybridization | 9739112 |
RLID00037043 | Germ plasm | Oocyte | In situ hybridization | 9739112 |
RLID00037044 | Germ plasm | Oocyte | In situ hybridization | 11784096 |
RLID00037045 | Mitochondrion | Oocyte | In situ hybridization | 11784096 |
RLID00037046 | Vegetal | Oocyte | In situ hybridization | 12676327 |
RLID00037047 | Mitochondrion | Oocyte | Quantitative RNA localization assay | 19223445 |