Detail
| RLID: |
RLID00037040 |
| RNA Symbol: |
Xlsirt |
| RNA Category: |
lncRNA |
| Organism: |
Xenopus laevis |
| Subcellular Localization: |
| Localization |
Tissue |
Validated Method |
PMID |
| Vegetal |
Oocyte |
In situ hybridization |
9545550 |
|
Description: To definitively demonstrate the co-localization of Vg1 mRNA with the wedge-shaped ER structure we performed in situ hybridization to identify the location of the Vg1 mRNA, and immunostaining with GRP-78 to identify the position of the ER. During stage 1, while Vg1 mRNA was dispersed throughout the cytoplasm, very little ER was associated with the cortex. (D) Stage 3 oocyte showing endogenous Xlsirts (in situ hybridization) localized at the vegetal cortex (blue) at the leading edge of the wedge-shaped ER (red) (the arrow is pointing to the Xlsirts at the leading edge of the ER). Note that by stage 2 the ER is also uniformly distributed throughout the entire cortical region. (E) Late stage 3 oocyte showing Xlsirts localized within the cortex (blue) and the remnants of the wedge containing ER (red).
|
Other Subcellular Localization: