Detail
| RLID: |
RLID00037507 |
| RNA Symbol: |
lnc-058 |
| RNA Category: |
lncRNA |
| Organism: |
Mus musculus |
| Subcellular Localization: |
| Localization |
Tissue |
Validated Method |
PMID |
| Nucleus |
C2C12 cell |
qRT-PCR |
25512605 |
|
Description: FIG 3: Subcellular distribution of the novel lncRNAs. Histograms show the quantifications of sqRT-PCR performed on RNA extracted from cytoplasmic (Cyt) and nuclear (N) fractions of myoblasts maintained under GM or DM (day 2) conditions. Results of a representative experiment are shown in Fig. S3 in the supplemental material. (A) Graphs show the cytoplasmic/nuclear partitioning of the lncRNAs, considering their expression conditions in GM or DM. The red shadow highlights the region of the graph occupied by lncRNAs with a bipartite subcellular distribution as defined by cytoplasmic/nuclear partitioning of control transcripts (see below). (B) Graphs show the quantification of the subcellular localization of control transcripts used to test the quality of the cytoplasmic/nuclear fractionation under GM or DM conditions: precursor (pre-Gapdh) and mature Gapdh mRNA, Rnu1-2 (U1) snRNA, and Snord55 (sno55). The red shadow defines the cytoplasmic/nuclear partition region in which no evident cytoplasmic/nuclear distribution can be assigned. Error bars represent standard deviations of data from multiple independent experiments.
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Other Subcellular Localization: