Detail
| RLID: | RLID00037514 |
| RNA Symbol: | lincRNA-uc003opf.1 |
| RNA Category: | lncRNA |
| Organism: | Homo sapiens |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Nucleus | Esophageal squamous cell | qRT-PCR | 23872665 |
| Description: To determine the cellular localization of lincRNA-uc003opf.1, we fractionated ESCC cell lines into nuclear and cytoplasmic fractions. We can thoroughly separate nucleus from cytoplasm, as indicated by the results showing that GAPDH mRNA was exclusively detected in the cytoplasmic fraction, whereas the transcriptional level of nucleus-retained U6 was predominantly found in the nuclear fraction. For EC9706 cell line, qRT-PCR analysis revealed that (mean +/- SD) 40.3 +/- 17.3% lincRNA-uc003opf.1 was detected in the nuclear fraction, and 59.7 +/- 17.3% resided in the cytoplasm fraction. We got the similar results in TE-1 cell line that 39.7 +/- 9.5% and 60.3 +/- 9.5% lincRNA-uc003opf.1 were detected in the nuclear fraction and the cytoplasm fraction, respectively (Figure 1A). | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00037513 | Cytoplasm | Esophageal squamous cell | qRT-PCR | 23872665 |