Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Ribosome |
HT1080 cell |
qRT-PCR |
25753659 |
|
Description: Figure S1: Polysomal gradient analysis. HT1080 cells were incubated under control (21% oxygen) or hypoxic (1% oxygen) conditions for up to 36 h as described in Figure 1. Shown are representative original RT-PCR data (30 cycles for beta-Actin and the external standard [extSt]; 35 cycles for the other genes) from pooled samples to visualize mRNA distribution following fractionation of sucrose gradients at control conditions (C) and 36 h of hypoxia (Hy). The external standard (a synthetic in vitro transcript) was diluted to appropriate concentration for qPCR and added directly after gradient fractionation and prior to RNA isolation as a technical control. The external standard served for fraction dependent normalization.
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Other Subcellular Localization:
| RLID |
Localization |
Tissue |
Validated Method |
PMID |