Notice: Undefined index: Gene_ID in D:\php\htdocs\wanglab\rnalocate\detail.php on line 5
Detail

Detail

RLID: RLID00018182
RNA Symbol: ACTB
RNA Category: mRNA
Organism: Homo sapiens
Aliase: BRWS1, PS1TP5BP1
Homology: ACTB (Gallus gallus), Actb (Mus musculus)
Subcellular Localization:
Localization Tissue Validated Method PMID
Ribosome HT1080 cell qRT-PCR 25753659
Description: Figure S1: Polysomal gradient analysis. HT1080 cells were incubated under control (21% oxygen) or hypoxic (1% oxygen) conditions for up to 36 h as described in Figure 1. Shown are representative original RT-PCR data (30 cycles for beta-Actin and the external standard [extSt]; 35 cycles for the other genes) from pooled samples to visualize mRNA distribution following fractionation of sucrose gradients at control conditions (C) and 36 h of hypoxia (Hy). The external standard (a synthetic in vitro transcript) was diluted to appropriate concentration for qPCR and added directly after gradient fractionation and prior to RNA isolation as a technical control. The external standard served for fraction dependent normalization.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID