Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Endoplasmic reticulum |
COS-7 cell |
Fluorescence in situ hybridization |
24019514 |
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Description: COS-7 cells were transfected with plasmids containing either ALPP, t-ftz, or the AF1, AF2 fusion constructs and allowed to express mRNA for 18-24 h. The cells were then treated with DMSO (Ctrl), puromycin (Puro), or HHT for 30 min and then extracted with either digitonin alone, or for puromycin-treated cells, with 20 mM EDTA. The cells were then fixed, stained for mRNA using specific FISH probes (ALPP probe for AF1, ftz probe for AF2), and imaged. FIGURE 2B: quantification of the fluorescence intensities of mRNA in the ER and nucleus. Data are collected from Figure 2.
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Other Subcellular Localization:
| RLID |
Localization |
Tissue |
Validated Method |
PMID |