Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Dendrite |
Neuron |
Fluorescence in situ hybridization |
18539120 |
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Description: Fig. 1 Fluorescence in situ hybridization (FISH) of hippocampal neurons. (a-d) Neurons from wild-type (WT, right panels) or FMRP-knockout (KO, left panels) were cultured for 10 days and either not stimulated ('unstim', upper panels') or stimulated with 50uM DHPG for 15 minutes ('DHPG' lower panels). Corresponding histograms showing dendritic quantification of FISH experiments are shown to the right. Results for WT (black bars) and KO (white bars) are labeled on the x-axis. Close-up images of dendritic signals are shown to the right (in same order). (a) MAP1b mRNA (n=15-16; *p<0.001 for unstimulated vs. DHPG in WT, P>0.25 for KO). (b)CaMKIIa mRNA (n=15-17; *p<0.01 for WT, p>0.2 for KO). There was no significant difference in CaMKIIa abundance of unstimulated neurons between WT and KO (n=15, p>0.1). (c) Beta-actin mRNA (n=11-13; p>0.2). Scale bars=12um. (d-e) FISH for other mRNA targets of FMRP in both WT (right image panels) and KO (left image panels). Representative images shown for unstimulated (upper panels; 'unstim' and stimulated (lower panels; DHPG, 50uM), and close-up images of dendrites are displayed above the histograms (in same order). Histograms (right) showing dendritic fluorescence quantification are shown for WT (green) and KO (red) using probes to (d) SAPAP4 (n=16-17; p<0.01 for WT, p>25 for KO) and (e) GABA-A receptor delta (n=13-15; *, ***p<0.01 for unstimulated vs. DHPG in WT and for DHPG in WT and KO, **p<0.05 for unstimulated vs. DHPG in KO). (c) Dendritic CaMKIIa mRNA localization in response to DHPG (11DIV). (d) Dendritic SAPAP4 mRNA localization in response to DHPG (11DIV).
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Other Subcellular Localization:
| RLID |
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PMID |