Detail
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Brain |
RT-PCR|Immunoprecipitation |
22945799 |
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Description: Fig. 5 a Detection of KIF1Bb in purified mouse synaptoneurosomes. KIF1Bb is detected in total lysates from the cortex (T; 5 lg total protein) and is enriched in a sample of synaptoneurosomes purified from the cortex (S; 5 lg). The analysis, by RT-PCR, revealed the presence of Arc (activity regulated cytoskeleton-associated protein) mRNA (Fig. 8c), a well-known, dendritically localized mRNA [43-45]. Interestingly, Calmodulin mRNA was also part of this complex. Both were specifically found in the KIF1Bb-immunoprecipitated complex, but not in the negative control reaction (Fig. 8c, compare lanes 2 and 3). The specific association of Arc and Calmodulin mRNAs with the KIF1Bb-containing complex is further supported by the absence of non-synaptically or other synaptically localized mRNAs such as eEIF1a and aCaMKII (Fig. 8c). These observations would suggest the participation of KIF1Bb in RNP particles in association with at least two dendritically targeted mRNAs, Arc and CaM.
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Other Subcellular Localization:
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PMID |