Detail
| RLID: | RLID00014094 |
| RNA Symbol: | P4HB |
| RNA Category: | mRNA |
| Organism: | Homo sapiens |
| Aliase: | CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB |
| Homology: | PDI1 (Saccharomyces cerevisiae ) |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Ribosome | HT1080 cell | qRT-PCR | 25753659 |
| Description: Figure S1: Polysomal gradient analysis. HT1080 cells were incubated under control (21% oxygen) or hypoxic (1% oxygen) conditions for up to 36 h as described in Figure 1. Shown are representative original RT-PCR data (30 cycles for beta-Actin and the external standard [extSt]; 35 cycles for the other genes) from pooled samples to visualize mRNA distribution following fractionation of sucrose gradients at control conditions (C) and 36 h of hypoxia (Hy). The external standard (a synthetic in vitro transcript) was diluted to appropriate concentration for qPCR and added directly after gradient fractionation and prior to RNA isolation as a technical control. The external standard served for fraction dependent normalization. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |