Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Ribosome |
J558 cell |
Northern blot |
12923260 |
|
Description: FIGURE 4. Steady-state mRNA partitioning between cytosolic and ER-bound polysomes. Total RNA was isolated from cytosol and ER membrane fractions of Jurkat and J558 cells, as indicated in Figure 1A. For each reaction, 10 æ¸g of RNA was incubated with one of eight [32P]-labeled oligonucleotide probes representing three different classes of genes, based on the type of protein encoded: cytosolic, ER membrane, and ER lumenal. The nuclease-protected RNA fragments from parallel reactions with cytosol and ER-derived RNA were purified and separated by denaturing acrylamide gel electrophoresis. Quantification was performed by phosphorimager analysis; photostimulated luminescence output (PSL) values are indicated. Digital images of the phosphorimager output are depicted. The data are collected from Figure 4.
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Other Subcellular Localization:
| RLID |
Localization |
Tissue |
Validated Method |
PMID |