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Detail

Detail

RLID: RLID00037040
RNA Symbol: Xlsirt
RNA Category: lncRNA
Organism: Xenopus laevis
Subcellular Localization:
Localization Tissue Validated Method PMID
Vegetal Oocyte In situ hybridization 9545550
Description: To definitively demonstrate the co-localization of Vg1 mRNA with the wedge-shaped ER structure we performed in situ hybridization to identify the location of the Vg1 mRNA, and immunostaining with GRP-78 to identify the position of the ER. During stage 1, while Vg1 mRNA was dispersed throughout the cytoplasm, very little ER was associated with the cortex. (D) Stage 3 oocyte showing endogenous Xlsirts (in situ hybridization) localized at the vegetal cortex (blue) at the leading edge of the wedge-shaped ER (red) (the arrow is pointing to the Xlsirts at the leading edge of the ER). Note that by stage 2 the ER is also uniformly distributed throughout the entire cortical region. (E) Late stage 3 oocyte showing Xlsirts localized within the cortex (blue) and the remnants of the wedge containing ER (red).

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID