Detail
| RLID: | RLID00027689 |
| RNA Symbol: | hsa-mir-425 |
| RNA Category: | miRNA |
| Organism: | Homo sapiens |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Exosome | Plasma | qRT-PCR | 24937531 |
| Description: For miRNome analysis, two homogeneous samples were created: one by pooling 2 æ¸L of RNA from exosomes of diabetic patients and the other by pooling RNA from exosomes of control subjects. After reverse transcription (mirCURY LNA universal cDNA synthesis kit; Exiqon), the reaction was performed on a serum/plasma focus miRNA PCR panel 384 well (V1.R) (Exiqon) using the ABI 7900HT (Applied Biosystems). The most stable miRNAs (miR-425 and miR-423-5p) were identified by NormFinder and GeNorm, validated on each sample, and used as normalizators for circulating miRNAs. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |