Detail
| RLID: | RLID00032283 |
| RNA Symbol: | rno-miR-138-5p |
| RNA Category: | miRNA |
| Organism: | Rattus norvegicus |
| Aliase: | rno-miR-138 |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Dendrite | Hippocampus | In situ hybridization|Northern blot | 19465924 |
| Description: One of the identified miRNAs, miR-138, is highly enriched in the brain, localized within dendrites and negatively regulates the size of dendritic spines in rat hippocampal neurons. The observed enrichment of several miRNAs in RNA preparations from synaptosomes was validated for selected candidates by Northern blot analysis (Fig. 2A). To monitor the subcellular localization of miRNAs identified in our screen, we performed in situ hybridization (ISH) in rat hippocampal neurons at 18 days in vitro (DIV) (Fig. 2B), using probes for miR-9, miR-218, miR-138 and miR-124 as a control (Fig. 2A, Suppl. Table 1). | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |